【佳学基因检测】口腔肿瘤细胞外泌体 miR-10b 基因检测分析靶向药物选择

靶向基因检测2万多重要性

挖掘在《肿瘤致病基因检测与转移潜能分析》，发现收录《Contrast Media Mol Imaging》在. 2022 Aug 16;2022:3188992.发表了一篇题目为《口腔肿瘤细胞外泌体 miR-10b 通过 AKT 信号传导刺激细胞侵袭和迁移》肿瘤靶向药物治疗基因检测临床研究文章。该研究由Xiang Li, Ting Yang, Chuanji Shu 等完成。促进了肿瘤的精准治疗与个性化用药的发展，进一步强调了基因信息检测与分析的重要性。

肿瘤靶向药物及精准治疗临床研究内容关键词：

口腔肿瘤,口腔癌细胞,外泌体,基因检测,靶向药物

肿瘤靶向治疗基因检测临床应用结果

已鉴定出源自癌细胞的外泌体可调节细胞间通讯。然而，口腔癌衍生的胞外域在肿瘤转移中的作用需要进一步研究。我们在本文中研究了它们在口腔癌细胞中的作用。对口腔癌细胞的强制作用主要归因于 miR-10b，一种在外泌体中具有高水平的基因，通过口腔癌衍生的外泌体转移到受体细胞。通过外泌体分离试剂获得外泌体。此外，通过电子显微镜进行外泌体鉴定和分析。通过qRT-PCR分析miRNA的表达。通过蛋白质印迹分析蛋白质表达。此外，还进行了侵袭和迁移实验以测定和评估外泌体 miR-10b 的功能。根据研究结果，外泌体介导的 miR-10b 转移促进了口腔癌细胞的行为。最后，发现AKT信号参与调节外泌体介导的口腔癌细胞侵袭和迁移，其激活降低了miR-10b敲低对口腔癌细胞的抑制作用。来自口腔癌细胞的外泌体 miR-10b 通过激活 AKT 信号传导增强细胞侵袭和迁移。

肿瘤发生与复发转移国际数据库描述：

An exosome derived from a cancer cell has been identified to regulate intercellular communication. However, the roles of oral cancer-derived ectodomains in tumor metastasis need to be investigated further. We investigated their roles in oral cancer cells in this paper. The enforcing effect on oral cancer cells was attributed primarily to miR-10b, a gene with a high level in exosomes that is transferred to recipient cells via oral cancer-derived exosomes. Exosomes were obtained by exosome isolation reagents. Also, exosome identification and analysis were performed by electron microscopy. The expression of miRNAs was analyzed by qRT-PCR. Protein expression was analyzed by Western blot. Also, invasion and migration experiments were performed to assay and evaluate the function of exosomal miR-10b. Exosome-mediated transfer of miR-10b promoted oral cancer cell behaviors, according to the findings. Finally, it was discovered that AKT signaling participates in regulating exosome-mediated invasion and migration of oral cancer cells and its activation reduced the inhibitory effect of miR-10b knockdown on oral cancer cells. Exosomal miR-10b derived from oral cancer cells enhances cell invasion and migration by activating AKT signaling.

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